: Figure Out the cycle at which the second spinoff of fluorescence with regard to cycle amount is maximal (Rasmussen, 2001). Community fitting to some parabola can be used to outline the peak situation with sub‐cycle precision. : To quantify the absolute range of RNA molecules for every sample, involve https://www.facebook.com/NewTechFind/posts/pfbid09GcrMB8SNQcBVaVb1McNe485n7DwQkzzJnGfi7W172g9g2PkhrJNxJzjejLgKFpRl